In the course of discussions about Enzyme Function arising from the companion blog, I became aware that DHFR Type 1 is described as a monomer. It is divided into two sub-domains. As this is inconsistent with the physics of catalysis (see the first post of this blog), I explored possible dimeric and tetrameric complexes of this enzyme. The tetramer docks very well. The tetrameric structure is now included in the gallery. Click on the title of this post to study the 3D structure.
I checked the sources of reports that DHFR Type 1 is a monomer and found that the direct evidence came from experiments in the absence of cofactor. I am now planning to repeat the MW determination in the presence of cofactor.
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